makes_use_of
type: ObjectProperty
type: TransitiveProperty
has OBO namespace: image
range: imaging method
domain: imaging method
has definition: The child is an essential component of the parent device, or plays an essential role in performance of the parent function.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

Is a
type: ObjectProperty
has OBO namespace: image

dummy term
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

sample preparation method
type: Class
subclass of: Biological Imaging Method
has OBO namespace: image

chemically fixed tissue
type: Class
subclass of: sample preparation method
disjoint with: unfixed tissue
has OBO namespace: image

coagulating-fixative fixed tissue
type: Class
subclass of: chemically fixed tissue
subclass of: solvent permeabilized
has OBO namespace: image

acetone fixed tissue
type: Class
subclass of: coagulating-fixative fixed tissue
has OBO namespace: image

acrolein fixed tissue
type: Class
subclass of: coagulating-fixative fixed tissue
has OBO namespace: image

ethanol fixed tissue
type: Class
subclass of: coagulating-fixative fixed tissue
has OBO namespace: image

methanol fixed tissue
type: Class
subclass of: coagulating-fixative fixed tissue
has OBO namespace: image

crosslinking-fixative fixed tissue
type: Class
subclass of: chemically fixed tissue
has OBO namespace: image

ethylene glycol-bis-succinimidyl succinate fixed tissue
type: Class
subclass of: crosslinking-fixative fixed tissue
has OBO namespace: image
has related synonym: EGS

formaldehyde fixed tissue
type: Class
subclass of: crosslinking-fixative fixed tissue
has OBO namespace: image

glutaraldehyde fixed tissue
type: Class
subclass of: crosslinking-fixative fixed tissue
has OBO namespace: image

osmium tetroxide fixed tissue
type: Class
subclass of: crosslinking-fixative fixed tissue
has OBO namespace: image

cryofixed tissue
type: Class
subclass of: sample preparation method
has OBO namespace: image

unfixed tissue
type: Class
subclass of: sample preparation method
has OBO namespace: image

embedded tissue
type: Class
subclass of: sample preparation method
disjoint with: unembedded tissue
has OBO namespace: image

tissue in plastic embedment
type: Class
subclass of: embedded tissue
has OBO namespace: image

tissue in acrylic resin embedment
type: Class
subclass of: tissue in plastic embedment
has OBO namespace: image

tissue in epoxy resin embedment
type: Class
subclass of: tissue in plastic embedment
has OBO namespace: image

tissue in wax embedment
type: Class
subclass of: embedded tissue
has OBO namespace: image

tissue in paraffin embedment
type: Class
subclass of: tissue in wax embedment
has OBO namespace: image

tissue in polyester wax embedment
type: Class
subclass of: tissue in wax embedment
has OBO namespace: image

tissue in polyethylene glycol embedment
type: Class
subclass of: tissue in wax embedment
has OBO namespace: image

unembedded tissue
type: Class
subclass of: sample preparation method
has OBO namespace: image

whole mounted tissue
type: Class
subclass of: sample preparation method
disjoint with: sectioned tissue
has OBO namespace: image

living tissue
type: Class
subclass of: unfixed tissue
has OBO namespace: image
has related synonym: live-cell imaging
has definition: Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state.
  type: Definition
  has db xref: XX:ISBN978-087969683-2
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_ISBN978-087969683-2

sectioned tissue
type: Class
subclass of: sample preparation method
has OBO namespace: image

cryostat-sectioned tissue
type: Class
subclass of: sectioned tissue
has OBO namespace: image

hand-sectioned tissue
type: Class
subclass of: sectioned tissue
has OBO namespace: image

microtome-sectioned tissue
type: Class
subclass of: sectioned tissue
has OBO namespace: image

vibratome-sectioned tissue
type: Class
subclass of: sectioned tissue
has OBO namespace: image

visualization method
type: Class
subclass of: Biological Imaging Method
has OBO namespace: image

endogenous substrate, non-vital dye
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

diagram
type: Class
subclass of: portrayed image
has OBO namespace: image

HA peptide tag
type: Class
subclass of: small genetically encoded tag
has OBO namespace: image

cresyl fast violet
type: Class
subclass of: optically-dense stain
has OBO namespace: image

endogenous substrate, diaminobenzidine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

eosin
type: Class
subclass of: non-specific protein affinity
has OBO namespace: image
has definition: Definition
  label: Staining with an aqueous or alcoholic solution of eosin Y,colors eosinophilic structures in various shades of red, pink and orange. The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and Mallory bodies are examples of eosinophilic structures. Most of the cytoplasm is eosinophilic. Red blood cells are stained intensely red.
  has db xref: Wikipedia:http://en.wikipedia.org/wiki/H%26E_stain
    type: DbXref
    has URI: http://purl.org/obo/owl/Wikipedia#Wikipedia_http://en.wikipedia.org/wiki/H%26E_stain

ethidium bromide
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image

fuchsin
type: Class
subclass of: optically-dense stain
has OBO namespace: image

giemsa
type: Class
subclass of: optically-dense stain
has OBO namespace: image

hematoxylin
type: Class
subclass of: non-specific protein affinity
has OBO namespace: image
has definition: Definition
  label: The staining method involves application of hemalum, which is a complex formed from aluminium ions and oxidized haematoxylin. This colors nuclei of cells (and a few other objects, such as keratohyalin granules) blue. The staining of nuclei by hemalum does not require the presence of DNA and is probably due to binding of the dye-metal complex to arginine-rich basic nucleoproteins such as histones. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes is prevented by chemical or enzymatic extraction of nucleic acids. Such extractions do not prevent staining of nuclei by hemalum.
  has db xref: Wikipedia:http://en.wikipedia.org/wiki/H%26E_stain
    type: DbXref
    has URI: http://purl.org/obo/owl/Wikipedia#Wikipedia_http://en.wikipedia.org/wiki/H%26E_stain

methyl green
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image

methyl violet
type: Class
subclass of: optically-dense stain
has OBO namespace: image

nitro blue tetrazolium chloride
type: Class
subclass of: optically-dense stain
has OBO namespace: image

nuclear fast red
type: Class
subclass of: optically-dense stain
has OBO namespace: image

orcein
type: Class
subclass of: non-specific protein affinity
has OBO namespace: image

propidium iodide
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image
has related synonym: PI

SYTOX Green
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image

toluidine blue
type: Class
subclass of: optically-dense stain
has OBO namespace: image

endogenous substrate, vital dye
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

acridine orange
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image
has related synonym: AO

Hoechst 33342
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image
has related synonym: HO342
has related synonym: Hoechst 33342

cationic colloidal gold
type: Class
subclass of: optically-dense stain
has OBO namespace: image

CMX rosamine (Mitotracker Red)
type: Class
subclass of: probe for mitochondria
has OBO namespace: image
has related synonym: Mitotracker X

DiOC6 (3,3'-dihexyloxacarbocyanine iodide)
type: Class
subclass of: probe for lipid
has OBO namespace: image
has related synonym: DiOC

4',6-diamidino-2-phenylindole (DAPI)
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image
has related synonym: DAPI

lucifer yellow
type: Class
subclass of: miscellaneous fill
has OBO namespace: image

methylene blue
type: Class
subclass of: optically-dense stain
has OBO namespace: image

Nile blue A
type: Class
subclass of: optically-dense stain
has OBO namespace: image

Nile red
type: Class
subclass of: probe for lipid
has OBO namespace: image

pyronine
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image

rhodamine 123
type: Class
subclass of: probe for mitochondria
has OBO namespace: image

rhodamine B
type: Class
subclass of: optically-dense stain
has OBO namespace: image

SYTO Blue
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image

SYTO Green
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image

SYTO Orange
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image

SYTO Red
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image

YO-PRO-1
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image

endogenous substrate, dye conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

endogenous substrate, acidotropic-fluor conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: LysoTracker

endogenous substrate, lectin-fluor conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

endogenous substrate, lectin-gold conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

taxol
type: Class
subclass of: affinity for specific proteins
has OBO namespace: image

endogenous substrate, phalloidin-fluor conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

exogenous label
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

genetically encoded enzyme
type: Class
subclass of: genetically encoded tag
has OBO namespace: image

beta-galactosidase
type: Class
subclass of: genetically encoded enzyme
has OBO namespace: image
has related synonym: lacZ, X-gal

beta-glucuronidase
type: Class
subclass of: genetically encoded enzyme
has OBO namespace: image
has related synonym: GUS, X-gluc

genetically encoded tag
type: Class
subclass of: macromolecular probe
has OBO namespace: image

EBFP
type: Class
subclass of: Blue fluorescent protein from Aequorea victoria
has OBO namespace: image
has related synonym: BFP

ECFP
type: Class
subclass of: Cyan fluorescent protein from Aequorea
has OBO namespace: image
has related synonym: CFP

EGFP
type: Class
subclass of: Green fluorescent protein from Aequorea
has OBO namespace: image
has related synonym: GFP

Aequorea victoria red fluorescent protein tag
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: RFP

EYFP
type: Class
subclass of: Yellow fluorescent protein from Aequorea
has OBO namespace: image
has related synonym: YFP

DsRed
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image
has related synonym: DsRed

c-MYC peptide tag
type: Class
subclass of: small genetically encoded tag
has OBO namespace: image

FLAG peptide tag
type: Class
subclass of: small genetically encoded tag
has OBO namespace: image

intracellular filling
type: Class
subclass of: visualization of contiguous regions
has OBO namespace: image
has definition: visualization of the connected regions of a cell by filling with a label
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

tissue in vitreous ice embedment
type: Class
subclass of: embedded tissue
has OBO namespace: image
has definition: Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C
  type: Definition
  has db xref: Adrian_M:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/Adrian_M#Adrian_M_NULL
  has db xref: PubMed:PMID:_3043536_"Cryo-electron_microscopy_of_vitrified_specimens._Dubochet_J
    type: DbXref
    has URI: http://purl.org/obo/owl/PubMed#PubMed_PMID:_3043536_"Cryo-electron_microscopy_of_vitrified_specimens._Dubochet_J
  has db xref: Lepault_J:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/Lepault_J#Lepault_J_NULL
  has db xref: McDowall_AW:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/McDowall_AW#McDowall_AW_NULL
  has db xref: Schultz_P._Q_Rev_Biophys._1988_May;21(2):129-228"
    type: DbXref
    has URI: http://purl.org/obo/owl/Schultz_P._Q_Rev_Biophys._1988_May#Schultz_P._Q_Rev_Biophys._1988_May;21(2)_129-228"
  has db xref: Chang_JJ:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/Chang_JJ#Chang_JJ_NULL
  has db xref: Homo_JC:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/Homo_JC#Homo_JC_NULL
has related synonym: tissue in amorphous ice embedment

fluor labeled RNA fill
type: Class
subclass of: intracellular filling
has OBO namespace: image

5-SFX fluorescein RNA fill
type: Class
subclass of: fluor labeled RNA fill
has OBO namespace: image

X-rhodamine RNA fill
type: Class
subclass of: fluor labeled RNA fill
has OBO namespace: image

permeabilized tissue
type: Class
subclass of: sample preparation method
has OBO namespace: image
has definition: Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

fluor labeled protein fill
type: Class
subclass of: intracellular filling
has OBO namespace: image

fluor conjugated actin fill
type: Class
subclass of: fluor labeled protein fill
has OBO namespace: image

Alexa Fluor 488 actin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

Alexa Fluor 568 actin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

fluoresceine actin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

rhodamine actin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

phalloidin
type: Class
subclass of: affinity for specific proteins
has OBO namespace: image

Alexa Fluor 350 phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

Alexa Fluor 488 phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

eosin phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

fluoresceine phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

Oregon Green 488 phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

Oregon Green 514 phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

rhodamine phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

Texas Red-X phalloidin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

fluor conjugated tubulin fill
type: Class
subclass of: fluor labeled protein fill
has OBO namespace: image

Oregon Green 514 tubulin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

X-rhodamine tubulin fill
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

probe for lipid
type: Class
subclass of: small-molecule probe
has OBO namespace: image

DiA
type: Class
subclass of: probe for lipid
has OBO namespace: image

DiD
type: Class
subclass of: probe for lipid
has OBO namespace: image

DiI
type: Class
subclass of: probe for lipid
has OBO namespace: image

DiO
type: Class
subclass of: probe for lipid
has OBO namespace: image

DiR
type: Class
subclass of: probe for lipid
has OBO namespace: image

miscellaneous fill
type: Class
subclass of: intracellular filling
has OBO namespace: image

biotin ethylenediamine fill
type: Class
subclass of: miscellaneous fill
has OBO namespace: image
has related synonym: Neurobiotin

cobalt fill
type: Class
subclass of: miscellaneous fill
has OBO namespace: image

silver-intensified cobalt fill
type: Class
subclass of: cobalt fill
has OBO namespace: image

metabolically incorporated radioisotope
type: Class
subclass of: visualization by chemical attribute
has OBO namespace: image

[14]C
type: Class
subclass of: metabolically incorporated radioisotope
has OBO namespace: image

[32]P
type: Class
subclass of: metabolically incorporated radioisotope
has OBO namespace: image

[33]P
type: Class
subclass of: metabolically incorporated radioisotope
has OBO namespace: image

[35]S
type: Class
subclass of: metabolically incorporated radioisotope
has OBO namespace: image

[3]H
type: Class
subclass of: metabolically incorporated radioisotope
has OBO namespace: image

label conjugated to probe
type: Class
subclass of: visualization method
has OBO namespace: image

glycerol permeabilized
type: Class
subclass of: permeabilized tissue
has OBO namespace: image
has definition: tissue made permeable by the action of glycerol
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref
has related synonym: glycerinated tissue

labeled primary antibody
type: Class
subclass of: antibody
has OBO namespace: image

primary antibody-enzyme conjugate, substrate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

alkaline phosphatase
type: Class
subclass of: enzyme label
has OBO namespace: image

primary antibody-alkaline phosphastase conjugate, DAB
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-alkaline phosphastase conjugate, Vector Black
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-alkaline phosphastase conjugate, Vector Blue
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-alkaline phosphastase conjugate, Vector Red
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

horseradish peroxidase
type: Class
subclass of: enzyme label
has OBO namespace: image

primary antibody-horseradish peroxidase, 4-chloro-1-naphthol
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-horseradish peroxidase, Vector NovaRed
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-horseradish peroxidase, Vector SG
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-horseradish peroxidase, Vector VIP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-fluor conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-aminomethylcoumarin acetate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: AMCA

primary antibody-Cascade blue
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody-cyanine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: Cy2

primary antibody-fluorescein isothiocyanate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: FITC

primary antibody-indocarbocyanine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: Cy3

primary antibody-indodicarbocyanine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: Cy5

primary antibody-rhodamine red-X
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: RRX

primary antibody-tetramethyl rhodamine isothiocyanate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: TRITC

primary antibody-Texas Red
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: TR

primary antibody-gold conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

primary antibody plus labeled secondary antibody
type: Class
subclass of: antibody
has OBO namespace: image

secondary antibody-enzyme conjugate, substrate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

acid phosphatase
type: Class
subclass of: enzyme label
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, DAB
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, Fast Blue B
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, Fast Red
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, INT
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, Magenta Phos
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, NABP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, NAGP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, NAMP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, NATP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, NBT
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, Vector Black
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, Vector Blue
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, Vector Red
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-alkaline phosphastase conjugate, BCIP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-beta-galactosidase, X-Gal
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-beta-glucuronidase, X-gluc
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

esterase
type: Class
subclass of: enzyme label
has OBO namespace: image

secondary antibody-esterase, Naphthol-AS-D-chloroacetate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

glucose oxidase
type: Class
subclass of: enzyme label
has OBO namespace: image

secondary antibody-glucose oxidase, TNBT
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine silver enhanced
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, Vector NovaRed
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, Vector SG
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-horseradish peroxidase, Vector VIP
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-phosphatase, Naphthol-AS-BI-phosphate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-fluor conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

secondary antibody-Alexa Fluor conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

aminomethylcoumarin
type: Class
subclass of: fluorescent label
has OBO namespace: image
has related synonym: AMCA

Cascade blue
type: Class
subclass of: fluorescent label
has OBO namespace: image

secondary antibody-cyanine conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: Cy2

secondary antibody-diaminotriazinylaminoflorescein conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: DTAF

secondary antibody-fluorescein isothiocyanate conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: FITC

secondary antibody-indocarbocyanine conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: Cy3

secondary antibody-indodicarbocyanine conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: Cy5

secondary antibody-lissamine rhodamine sulfonyl chloride
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: LSRC

secondary antibody-rhodamine red-X conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: RRX

secondary antibody-tetramethyl rhodamine isothiocyanate conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: TRITC

secondary antibody-Texas red conjugate
type: Class
subclass of: ObsoleteClass
has OBO namespace: image
has related synonym: TR

gold
type: Class
subclass of: electron dense label
has OBO namespace: image

labeled nucleic acid probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

DNA probe
type: Class
subclass of: sequence-specific nucleic acid probe
has OBO namespace: image

biotin labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

digoxigenin labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

fluor labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

BODIPY TR-14-dUTP labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

bromodeoxyuridine labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

fluoresceine-12-dUTP labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

Oregon Green 488-5-dUTP labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

radioisotope labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

[32]P labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

[33]P labeled DNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

RNA probe
type: Class
subclass of: sequence-specific nucleic acid probe
has OBO namespace: image

biotin labeled RNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

digoxigenin labeled RNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

fluoroscein labeled RNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

radioisotope labeled RNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

[32]P labeled RNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

[33]P labeled RNA probe
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

imaging method
type: Class
subclass of: Biological Imaging Method
has OBO namespace: image

graphic illustration
type: Class
subclass of: portrayed image
has OBO namespace: image

computer graphic
type: Class
subclass of: graphic illustration
has OBO namespace: image

black and white graphic
type: Class
subclass of: computer graphic
has OBO namespace: image

color graphic
type: Class
subclass of: computer graphic
has OBO namespace: image

grey scale graphic
type: Class
subclass of: computer graphic
has OBO namespace: image

camera lucida assisted graphic
type: Class
subclass of: graphic illustration
has OBO namespace: image

free hand graphic
type: Class
subclass of: graphic illustration
has OBO namespace: image

line art graphic
type: Class
subclass of: graphic illustration
has OBO namespace: image

charcoal pencil graphic
type: Class
subclass of: line art graphic
has OBO namespace: image

colored pencil graphic
type: Class
subclass of: line art graphic
has OBO namespace: image

graphite pencil graphic
type: Class
subclass of: line art graphic
has OBO namespace: image

pen and ink graphic
type: Class
subclass of: line art graphic
has OBO namespace: image
has related synonym: black and white graphic

painted graphic
type: Class
subclass of: line art graphic
has OBO namespace: image

acrylic painted graphic
type: Class
subclass of: painted graphic
has OBO namespace: image

oil painted graphic
type: Class
subclass of: painted graphic
has OBO namespace: image

pastel painted graphic
type: Class
subclass of: painted graphic
has OBO namespace: image

watercolor painted graphic
type: Class
subclass of: painted graphic
has OBO namespace: image

macroscopy
type: Class
subclass of: recorded image
has OBO namespace: image
has definition: methods for imaging objects large enough to be observed by the unaided eye
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

microscopy
type: Class
subclass of: recorded image
has OBO namespace: image
has definition: methods for forming images of objects too small to be observed with the unaided eye
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

wide field light micrograph
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

bright-field microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: widefield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: wide-field detection
  intersection of: Restriction
    on property: makes_use_of
    some values from: absorption of illumination
subclass of: mode of light microscopy
has OBO namespace: image

dark-field microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: darkfield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: elastic scattering
subclass of: mode of light microscopy
has OBO namespace: image
has definition: imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

differential interference contrast microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: optical path length gradient
  intersection of: Restriction
    on property: makes_use_of
    some values from: Wollaston prism
  intersection of: Restriction
    on property: makes_use_of
    some values from: polarized light illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: polarization-sensitive detection
subclass of: mode of light microscopy
has OBO namespace: image
has related synonym: Nomarski
has related synonym: DIC

fluorescence microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: fluorescence emission
  intersection of: Restriction
    on property: makes_use_of
    some values from: UV/visible/IR illumination
subclass of: mode of light microscopy
has OBO namespace: image

phase contrast microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: hollow-cone illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: refractive index
subclass of: mode of light microscopy
has OBO namespace: image

polarization microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: retardance
  intersection of: Restriction
    on property: makes_use_of
    some values from: polarized light illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: polarization-sensitive detection
subclass of: mode of light microscopy
has OBO namespace: image

time lapse microscopy
type: Class
subclass of: mode of light microscopy
has OBO namespace: image

narrow field light micrograph
type: Class
subclass of: ObsoleteClass
has OBO namespace: image

confocal microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrowfield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrow-field detection
  intersection of: Restriction
    on property: makes_use_of
    some values from: fluorescence emission
subclass of: mode of light microscopy
has OBO namespace: image

single-spot confocal microscopy
type: Class
equivalent class: Class
  intersection of: confocal microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: single point scanning
  intersection of: Restriction
    on property: makes_use_of
    some values from: spot detector
subclass of: confocal microscopy
has OBO namespace: image

spinning disk confocal microscopy
type: Class
equivalent class: Class
  intersection of: confocal microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: spinning disk scanning
  intersection of: Restriction
    on property: makes_use_of
    some values from: charge coupled device (CCD)
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrow-field detection
subclass of: confocal microscopy
has OBO namespace: image

two-photon laser scanning microscopy
type: Class
subclass of: multi-photon microscopy
has OBO namespace: image

multi-photon microscopy
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrowfield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: pulsed illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: fluorescence emission
subclass of: mode of light microscopy
has OBO namespace: image

mode of electron microscopy
type: Class
equivalent class: Class
  intersection of: microscopy with lenses
  intersection of: Restriction
    on property: makes_use_of
    some values from: illumination by electrons
subclass of: microscopy with lenses
has OBO namespace: image

scanning electron microscopy (SEM)
type: Class
equivalent class: Class
  intersection of: mode of electron microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrowfield illumination
subclass of: mode of electron microscopy
has OBO namespace: image
has definition: image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

transmission electron microscopy (TEM)
type: Class
equivalent class: Class
  intersection of: mode of electron microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: widefield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: wide-field detection
subclass of: mode of electron microscopy
has OBO namespace: image

atomic force microscopy
type: Class
subclass of: scanning probe microscopy
has OBO namespace: image
has definition: the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

X-ray micrograph
type: Class
subclass of: X-ray illumination
has OBO namespace: image

montage
type: Class
subclass of: portrayed image
has OBO namespace: image

detergent permeabilized
type: Class
subclass of: permeabilized tissue
has OBO namespace: image
has definition: Membrane permeabilization by the action of ionic or non-ionic detergents
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

solvent permeabilized
type: Class
subclass of: permeabilized tissue
has OBO namespace: image
has definition: permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol.
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

saponin permeabilized
type: Class
subclass of: permeabilized tissue
has OBO namespace: image
has definition: Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol.
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>
has related synonym: saponized tissue

recorded image
type: Class
subclass of: imaging method
has OBO namespace: image
has related synonym: photograph
has related synonym: micrograph
has definition: methods and devices used for capturing an image of a real object; distinct from images created by artwork
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

portrayed image
type: Class
subclass of: imaging method
has OBO namespace: image
has related synonym: drawing
has related synonym: artwork
has definition: methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

animation
type: Class
subclass of: computer graphic
has OBO namespace: image
has definition: A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

illumination method
type: Class
subclass of: microscopy with lenses
has OBO namespace: image
has definition: sources and methods for illumination
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

detection method
type: Class
subclass of: microscopy with lenses
has OBO namespace: image
has definition: media and devices employed for recording the alterations in the illumination that result from its interaction with the sample
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

imaged parameter
type: Class
subclass of: microscopy with lenses
has OBO namespace: image
has definition: The physical property of the specimen that leads to the modifications of the illumination that are recorded in the image.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

contrast-enhancing method
type: Class
subclass of: microscopy with lenses
has OBO namespace: image

illumination by photons
type: Class
subclass of: illumination method
has OBO namespace: image

illumination by electrons
type: Class
subclass of: illumination method
has OBO namespace: image

illumination by acoustic waves
type: Class
subclass of: illumination method
has OBO namespace: image

illumination by neutrons
type: Class
subclass of: illumination method
has OBO namespace: image

illumination by ions
type: Class
subclass of: illumination method
has OBO namespace: image

widefield illumination
type: Class
subclass of: illumination method
has OBO namespace: image
has definition: illumination that covers the entire field of view of the image-forming lens
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

narrowfield illumination
type: Class
subclass of: illumination method
has OBO namespace: image
has definition: illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

coherent illumination
type: Class
subclass of: illumination method
has OBO namespace: image

incoherent illumination
type: Class
subclass of: illumination method
has OBO namespace: image

nearfield illumination
type: Class
subclass of: illumination method
has OBO namespace: image

farfield illumination
type: Class
subclass of: illumination method
has OBO namespace: image

continuous illumination
type: Class
subclass of: illumination method
has OBO namespace: image

pulsed illumination
type: Class
subclass of: illumination method
has OBO namespace: image
has definition: illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

oblique illumination
type: Class
subclass of: illumination method
has OBO namespace: image

single point scanning
type: Class
subclass of: narrowfield illumination
has OBO namespace: image
has definition: illumination by a single spot of light that is scanned across the field of view
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

multiple point scanning
type: Class
subclass of: narrowfield illumination
has OBO namespace: image
has definition: illumination by multiple points of light simultaneously
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

spinning disk scanning
type: Class
subclass of: multiple point scanning
has OBO namespace: image
has definition: scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

array scanning
type: Class
subclass of: multiple point scanning
has OBO namespace: image
has definition: Multiple points of illumination generated by a fixed array of sources.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

hollow-cone illumination
type: Class
subclass of: oblique illumination
has OBO namespace: image
has definition: illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

anaxial illumination
type: Class
subclass of: oblique illumination
has OBO namespace: image
has definition: Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser.
  type: Definition
  has db xref: NULL:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/NULL#NULL_NULL

darkfield illumination
type: Class
subclass of: hollow-cone illumination
has OBO namespace: image
has definition: illumination restricted to angles that exceed the acceptance angle of the image-forming lens
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

Rheinberg illumination
type: Class
subclass of: hollow-cone illumination
has OBO namespace: image
has definition: Definition
  label: illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

charge coupled device (CCD)
type: Class
subclass of: area detector
has OBO namespace: image

photomultiplier tube (PMT)
type: Class
subclass of: spot detector
has OBO namespace: image

photodiode
type: Class
subclass of: spot detector
has OBO namespace: image

avalanche photodiode (APD)
type: Class
subclass of: spot detector
has OBO namespace: image

silicon intensified target tube (SIT)
type: Class
subclass of: area detector
has OBO namespace: image

intensified SIT (ISIT)
type: Class
subclass of: area detector
has OBO namespace: image

intensified CCD (ICCD)
type: Class
subclass of: area detector
has OBO namespace: image

electron multiplying CCD (EMCCD)
type: Class
subclass of: area detector
has OBO namespace: image

electron bombardment CCD (EBCCD)
type: Class
subclass of: area detector
has OBO namespace: image

film
type: Class
subclass of: area detector
has OBO namespace: image

complementary metal oxide semiconductor (CMOS)
type: Class
subclass of: area detector
has OBO namespace: image

piezo-electric device
type: Class
subclass of: spot detector
has OBO namespace: image

wide-field detection
type: Class
subclass of: detection method
has OBO namespace: image

narrow-field detection
type: Class
subclass of: detection method
has OBO namespace: image

absorption of illumination
type: Class
subclass of: imaged parameter
has OBO namespace: image

inelastic scattering
type: Class
subclass of: imaged parameter
has OBO namespace: image

elastic scattering
type: Class
subclass of: imaged parameter
has OBO namespace: image

refractive index
type: Class
subclass of: imaged parameter
has OBO namespace: image

optical path length gradient
type: Class
subclass of: imaged parameter
has OBO namespace: image

retardance
type: Class
subclass of: imaged parameter
has OBO namespace: image

stiffness
type: Class
subclass of: imaged parameter
has OBO namespace: image

electron density
type: Class
subclass of: imaged parameter
has OBO namespace: image

fluorescence emission
type: Class
subclass of: imaged parameter
has OBO namespace: image

sub-nanosecond time-resolved fluorescence emission)
type: Class
subclass of: fluorescence emission
has OBO namespace: image

fluorescence polarization (polarization angle resolved fluorescence emission)
type: Class
subclass of: fluorescence emission
has OBO namespace: image

chemical composition
type: Class
subclass of: imaged parameter
has OBO namespace: image

elevation
type: Class
subclass of: imaged parameter
has OBO namespace: image

resolution-enhancing method
type: Class
subclass of: microscopy with lenses
has OBO namespace: image
has definition: methods for increasing the resolution beyond the classical Abbe diffraction limit
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

optical method
type: Class
subclass of: contrast-enhancing method
has OBO namespace: image

computational method
type: Class
subclass of: contrast-enhancing method
has OBO namespace: image

in-focus phase contrast
type: Class
subclass of: modified objective lens
has OBO namespace: image

defocus phase contrast
type: Class
subclass of: optical method
has OBO namespace: image

Wollaston prism
type: Class
subclass of: optical method
has OBO namespace: image
has definition: a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

Hoffman modulation
type: Class
subclass of: modified objective lens
has OBO namespace: image

single sideband edge enhancement (SSBE)
type: Class
subclass of: modified objective lens
has OBO namespace: image

interference reflection contrast (IRM)
type: Class
subclass of: optical method
has OBO namespace: image

interference contrast
type: Class
subclass of: optical method
has OBO namespace: image

polarization contrast
type: Class
subclass of: optical method
has OBO namespace: image

structured illumination microscopy (SIM)
type: Class
subclass of: resolution-enhancing method
has OBO namespace: image

point-localization method
type: Class
subclass of: resolution-enhancing method
has OBO namespace: image

stimulated emission depletion (STED)
type: Class
equivalent class: Class
  intersection of: resolution-enhancing method
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrowfield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: emission-depletion illumination
subclass of: resolution-enhancing method
has OBO namespace: image

PALM (photoactivation localization microscopy)
type: Class
equivalent class: Class
  intersection of: point-localization method
  intersection of: Restriction
    on property: makes_use_of
    some values from: photo-activation illumination
subclass of: point-localization method
has OBO namespace: image

STORM (stochastic optical reconstruction microscopy)
type: Class
equivalent class: Class
  intersection of: point-localization method
  intersection of: Restriction
    on property: makes_use_of
    some values from: photo-switching illumination
subclass of: point-localization method
has OBO namespace: image

FIONA (fluorescence imaging with one nanometer accuracy)
type: Class
subclass of: point-localization method
has OBO namespace: image

DOPI (defocussed orientation and position imaging)
type: Class
subclass of: point-localization method
has OBO namespace: image

PAINT (point accumulation for imaging in nanoscale topography)
type: Class
subclass of: point-localization method
has OBO namespace: image

saturated structured-illumination microscopy (SSIM)
type: Class
subclass of: structured illumination microscopy (SIM)
has OBO namespace: image
has definition: structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

UV/visible/IR illumination
type: Class
subclass of: illumination by photons
has OBO namespace: image

X-ray illumination
type: Class
subclass of: illumination by photons
has OBO namespace: image

microscopy with lenses
type: Class
subclass of: microscopy
has OBO namespace: image

scanning probe microscopy
type: Class
subclass of: microscopy
has OBO namespace: image
has definition: imaging by means of a physical probe that passes over the sample
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

mode of light microscopy
type: Class
equivalent class: Class
  intersection of: microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: UV/visible/IR illumination
subclass of: microscopy with lenses
has OBO namespace: image

scanning tunneling microscopy
type: Class
subclass of: scanning probe microscopy
has OBO namespace: image

nearfield scanning optical microscopy (ANSOM)
type: Class
subclass of: scanning probe microscopy
has OBO namespace: image

ANSOM
type: Class
subclass of: nearfield scanning optical microscopy (ANSOM)
has OBO namespace: image
has definition: Aperture-less NSOM.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

polarized light illumination
type: Class
subclass of: illumination by photons
has OBO namespace: image

orientation-independent polarization microscopy
type: Class
subclass of: polarization microscopy
has OBO namespace: image

polarization-sensitive detection
type: Class
subclass of: detection method
has OBO namespace: image

modified objective lens
type: Class
subclass of: optical method
has OBO namespace: image
has definition: Uses an objective with extra components to manipulate the wave front in the plane of the objective aperture.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

linear method
type: Class
subclass of: computational method
disjoint with: non-linear method
has OBO namespace: image
has definition: a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

non-linear method
type: Class
subclass of: computational method
has OBO namespace: image
has definition: computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

Wiener filter
type: Class
subclass of: linear method
has OBO namespace: image

3D-deconvolution
type: Class
subclass of: linear method
has OBO namespace: image

no-neighbors deblurring
type: Class
subclass of: non-linear method
has OBO namespace: image

nearest-neighbors deblurring
type: Class
subclass of: non-linear method
has OBO namespace: image

constrained iterative deconvolution
type: Class
subclass of: 3D-deconvolution
has OBO namespace: image

maximum likelihood deconvolution
type: Class
subclass of: 3D-deconvolution
has OBO namespace: image

blind deconvolution
type: Class
subclass of: 3D-deconvolution
has OBO namespace: image

exhaustive photon reassignment
type: Class
subclass of: 3D-deconvolution
has OBO namespace: image

light-sheet illumination
type: Class
subclass of: oblique illumination
has OBO namespace: image
has definition: illumination in the form of a thin sheet of light directed perpendicular to the optic axis
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

FLIP
type: Class
equivalent class: Class
  intersection of: fluorescence microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: photo-bleaching illumination
subclass of: fluorescence microscopy
has OBO namespace: image
has definition: Definition
  label: Fluorescence loss in photobleaching; a method of determining whether two populations of fluorophore are in diffusive communication with one another by observing the decrease in brightness of one population when the other population is subjected to continuous photobleaching.
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

FRAP
type: Class
equivalent class: Class
  intersection of: fluorescence microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: narrowfield illumination
  intersection of: Restriction
    on property: makes_use_of
    some values from: photo-bleaching illumination
subclass of: fluorescence microscopy
has OBO namespace: image
has definition: Definition
  label: Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching.
  has db xref: XX:-new-dbxref-
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_-new-dbxref-

FRET
type: Class
subclass of: fluorescence microscopy
has OBO namespace: image
has definition: Definition
  label: Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other.
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

FLIM
type: Class
subclass of: fluorescence microscopy
has OBO namespace: image

SPIM
type: Class
equivalent class: Class
  intersection of: fluorescence microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: light-sheet illumination
subclass of: fluorescence microscopy
has OBO namespace: image
has definition: single (or selective) plane illumination.
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

CARS
type: Class
equivalent class: Class
  intersection of: mode of light microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: inelastic scattering
  intersection of: Restriction
    on property: makes_use_of
    some values from: chemical composition
subclass of: mode of light microscopy
has OBO namespace: image
has definition: coherent Raman anti-Stokes microscopy
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

OCT
type: Class
subclass of: mode of light microscopy
has OBO namespace: image
has definition: optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

secondary_electron imaging
type: Class
subclass of: scanning electron microscopy (SEM)
has OBO namespace: image
has definition: image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

back-scattered_electron imaging
type: Class
equivalent class: Class
  intersection of: mode of electron microscopy
subclass of: scanning electron microscopy (SEM)
has OBO namespace: image
has definition: imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

detection of photons
type: Class
subclass of: detection method
has OBO namespace: image

detection of electrons
type: Class
subclass of: detection method
has OBO namespace: image

detection of acoustic waves
type: Class
subclass of: detection method
has OBO namespace: image

detection of current induced in the specimen
type: Class
subclass of: detection method
has OBO namespace: image

UV/visible/IR photons
type: Class
subclass of: detection of photons
has OBO namespace: image

X-ray photons
type: Class
subclass of: detection of photons
has OBO namespace: image

scanning-transmission electron microscopy
type: Class
subclass of: mode of electron microscopy
has OBO namespace: image

EELS imaging
type: Class
subclass of: mode of electron microscopy
has OBO namespace: image
has related synonym: EFTEM energy filtered TEM
has related synonym: ESI electron spectroscopic imaging

EDAX imaging
type: Class
subclass of: mode of electron microscopy
has OBO namespace: image

specimen_modifying_illumination
type: Class
subclass of: illumination method
has OBO namespace: image
has definition: Definition
  label: Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

photo-bleaching illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

photo-ablation illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

photo-switching illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

photo-activation illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

uncaging illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

trapping illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

emission-depletion illumination
type: Class
subclass of: specimen_modifying_illumination
has OBO namespace: image

slit-scanning illumination
type: Class
subclass of: narrowfield illumination
has OBO namespace: image
has definition: illumination in the shape of a thin line that is swept over the field of view
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

slit-scan confocal
type: Class
equivalent class: Class
  intersection of: confocal microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: slit-scanning illumination
subclass of: confocal microscopy
has OBO namespace: image
has definition: illumination and detection via a thin slit
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

array-scan confocal
type: Class
equivalent class: Class
  intersection of: confocal microscopy
  intersection of: Restriction
    on property: makes_use_of
    some values from: array scanning
  intersection of: Restriction
    on property: makes_use_of
    some values from: charge coupled device (CCD)
subclass of: confocal microscopy
has OBO namespace: image
has definition: Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

spot detector
type: Class
subclass of: detection method
has OBO namespace: image

area detector
type: Class
subclass of: detection method
has OBO namespace: image

visualization of contiguous regions
type: Class
subclass of: visualization method
has OBO namespace: image
has definition: methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

visualization by chemical attribute
type: Class
subclass of: visualization method
has OBO namespace: image
has definition: methods of visualization that differentiate regions of the specimen on the basis of their chemical properties
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

shadowing and plating
type: Class
subclass of: visualization of contiguous regions
subclass of: Restriction
  on property: makes_use_of
  some values from: freeze_fracture/freeze_etch
has OBO namespace: image
has definition: visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

negative staining
type: Class
subclass of: visualization of contiguous regions
has OBO namespace: image
has definition: visualization by exclusion of a stain in which the specimen is immersed; compare with "positive staining.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

sequence-specific nucleic acid probe
type: Class
subclass of: macromolecular probe
has OBO namespace: image

antibody
type: Class
subclass of: macromolecular probe
has OBO namespace: image

lectin
type: Class
subclass of: macromolecular probe
has OBO namespace: image

small-molecule probe
type: Class
subclass of: visualization by chemical attribute
has OBO namespace: image

fluorescent dextran fill
type: Class
subclass of: intracellular filling
has OBO namespace: image

fluorescent protein tag
type: Class
subclass of: genetically encoded tag
has OBO namespace: image

probes for nucleic acid
type: Class
subclass of: small-molecule probe
has OBO namespace: image

small genetically encoded tag
type: Class
subclass of: genetically encoded tag
has OBO namespace: image

probe for protein
type: Class
subclass of: small-molecule probe
has OBO namespace: image

organelle-specific probe
type: Class
subclass of: small-molecule probe
has OBO namespace: image

macromolecular probe
type: Class
subclass of: visualization by chemical attribute
has OBO namespace: image

probe for mitochondria
type: Class
subclass of: organelle-specific probe
has OBO namespace: image

probe for endoplasmic reticulum
type: Class
subclass of: organelle-specific probe
has OBO namespace: image

probe for Golgi
type: Class
subclass of: organelle-specific probe
has OBO namespace: image

probe for lysosomes
type: Class
subclass of: organelle-specific probe
has OBO namespace: image

stain with broad specificity
type: Class
subclass of: small-molecule probe
has OBO namespace: image
has related synonym: positive staining

tetracysteine tag
type: Class
subclass of: small genetically encoded tag
has OBO namespace: image

Hoechst 33258
type: Class
subclass of: membrane-permeant probe
has OBO namespace: image

genetically encoded biotin tag
type: Class
subclass of: small genetically encoded tag
has OBO namespace: image

freeze_fracture/freeze_etch
type: Class
equivalent class: Class
  intersection of: cryofixed tissue
subclass of: cryofixed tissue
has OBO namespace: image

fluorescent protein derived from Aequorea victoria
type: Class
subclass of: fluorescent protein tag
has OBO namespace: image

membrane-permeant probe
type: Class
subclass of: probes for nucleic acid
has OBO namespace: image

membrane impermeant probe
type: Class
subclass of: probes for nucleic acid
has OBO namespace: image

acridine homodimer
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image

7-amino-actinomycin D
type: Class
subclass of: membrane impermeant probe
has OBO namespace: image

conconavalin A
type: Class
subclass of: lectin
has OBO namespace: image

wheat germ agglutinin
type: Class
subclass of: lectin
has OBO namespace: image

Phaseolus vulgaris lectin PHA-L)
type: Class
subclass of: lectin
has OBO namespace: image

Arachis hypogaea (PNA)
type: Class
subclass of: lectin
has OBO namespace: image

Helix pomatia agglutinin
type: Class
subclass of: lectin
has OBO namespace: image

soybean agglutinin
type: Class
subclass of: lectin
has OBO namespace: image

cholera toxin B
type: Class
subclass of: lectin
has OBO namespace: image

peptide-nucleic acid probe
type: Class
subclass of: sequence-specific nucleic acid probe
has OBO namespace: image

fluorescent protein derived from Anthozoa
type: Class
subclass of: fluorescent protein tag
has OBO namespace: image

optical highlighter
type: Class
subclass of: fluorescent protein tag
has OBO namespace: image

Blue fluorescent protein from Aequorea victoria
type: Class
subclass of: fluorescent protein derived from Aequorea victoria
has OBO namespace: image

Cyan fluorescent protein from Aequorea
type: Class
subclass of: fluorescent protein derived from Aequorea victoria
has OBO namespace: image

Green fluorescent protein from Aequorea
type: Class
subclass of: fluorescent protein derived from Aequorea victoria
has OBO namespace: image

DiIC16
type: Class
subclass of: probe for lipid
has OBO namespace: image

Yellow fluorescent protein from Aequorea
type: Class
subclass of: fluorescent protein derived from Aequorea victoria
has OBO namespace: image

Alexa Fluor 488
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 546
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 568
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 555
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 594
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 610
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 633
type: Class
subclass of: fluorescent label
has OBO namespace: image

Alexa Fluor 647
type: Class
subclass of: fluorescent label
has OBO namespace: image

Cy2
type: Class
subclass of: fluorescent label
has OBO namespace: image

Cy3
type: Class
subclass of: fluorescent label
has OBO namespace: image

Cy5
type: Class
subclass of: fluorescent label
has OBO namespace: image

Fluorescein (FITC)
type: Class
subclass of: fluorescent label
has OBO namespace: image

Rhodamine
type: Class
subclass of: fluorescent label
has OBO namespace: image

TexasRed
type: Class
subclass of: fluorescent label
has OBO namespace: image
has definition: fluorophore with ex/em 589/615
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

Tetramethyl rhodamine (TRITC)
type: Class
subclass of: fluorescent label
has OBO namespace: image
has definition: fluorophore derivative of rhodamine with ex/em 547/572
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

fluorescent label
type: Class
subclass of: label conjugated to probe
has OBO namespace: image

enzyme label
type: Class
subclass of: label conjugated to probe
has OBO namespace: image

electron dense label
type: Class
subclass of: label conjugated to probe
has OBO namespace: image

biotin
type: Class
subclass of: fluorescent label
has OBO namespace: image

affinity for specific proteins
type: Class
subclass of: probe for protein
has OBO namespace: image

non-specific protein affinity
type: Class
subclass of: probe for protein
has OBO namespace: image

AzuriteFP
type: Class
subclass of: Blue fluorescent protein from Aequorea victoria
has OBO namespace: image

SiriusFP
type: Class
subclass of: Blue fluorescent protein from Aequorea victoria
has OBO namespace: image

non-immunological protein probe
type: Class
subclass of: macromolecular probe
has OBO namespace: image

alpha-bungarotoxin
type: Class
subclass of: non-immunological protein probe
has OBO namespace: image

botulinum toxin
type: Class
subclass of: non-immunological protein probe
has OBO namespace: image

tetrodotoxin
type: Class
subclass of: affinity for specific proteins
has OBO namespace: image

batrachatoxin
type: Class
subclass of: affinity for specific proteins
has OBO namespace: image

CeruleanFP
type: Class
subclass of: Cyan fluorescent protein from Aequorea
has OBO namespace: image

CyPet
type: Class
subclass of: Cyan fluorescent protein from Aequorea
has OBO namespace: image

SCFP
type: Class
subclass of: Cyan fluorescent protein from Aequorea
has OBO namespace: image

EmeraldFP
type: Class
subclass of: Green fluorescent protein from Aequorea
has OBO namespace: image

SuperfolderFP
type: Class
subclass of: Green fluorescent protein from Aequorea
has OBO namespace: image

T-SapphireFP
type: Class
subclass of: Green fluorescent protein from Aequorea
has OBO namespace: image

CitrineFP
type: Class
subclass of: Yellow fluorescent protein from Aequorea
has OBO namespace: image

SYFP
type: Class
subclass of: Yellow fluorescent protein from Aequorea
has OBO namespace: image

TopazFP
type: Class
subclass of: Yellow fluorescent protein from Aequorea
has OBO namespace: image

VenusFP
type: Class
subclass of: Yellow fluorescent protein from Aequorea
has OBO namespace: image

yPet
type: Class
subclass of: Yellow fluorescent protein from Aequorea
has OBO namespace: image

Blue fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

Cyan fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

Green fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

Orange fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

Red fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

Far-red fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

Yellow fluorescent proteins from Anthozoa
type: Class
subclass of: fluorescent protein derived from Anthozoa
has OBO namespace: image

mTagBFP
type: Class
subclass of: Blue fluorescent proteins from Anthozoa
has OBO namespace: image

AmCyanFP
type: Class
subclass of: Cyan fluorescent proteins from Anthozoa
has OBO namespace: image

mCyFP
type: Class
subclass of: Cyan fluorescent proteins from Anthozoa
has OBO namespace: image

mTFP1
type: Class
subclass of: Cyan fluorescent proteins from Anthozoa
has OBO namespace: image

TagCFP
type: Class
subclass of: Cyan fluorescent proteins from Anthozoa
has OBO namespace: image

AQ143
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

HcRed-tandemFP
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

KatushkaFP
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

mKateFP
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

mKate2FP
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

mPlumFP
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

tdKatuskaFP
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

tdRFP639
type: Class
subclass of: Far-red fluorescent proteins from Anthozoa
has OBO namespace: image

AceFP
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

Azami GreenFP
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

CopGFP
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

mWasabiFP
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

TagGFP
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

TagGFP2
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

ZsGreenFP
type: Class
subclass of: Green fluorescent proteins from Anthozoa
has OBO namespace: image

DsRed-ExpressFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

DsRed-Express2FP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

DsRed-MaxFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

DsRed-monomerFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

DsRed2FP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

dTomatoFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

Kusabira OrangeFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

Kusabira OrangeFP2
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

mOrangeFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

mOrange2FP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

TagRFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

TagRFP-T
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

tdTomatoFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

turboRFP
type: Class
subclass of: Orange fluorescent proteins from Anthozoa
has OBO namespace: image

AsRed2FP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

eqFP611
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

HcRed1FP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

JRedFP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

mAppleFP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

mCherryFP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

mRasberryFP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

mRFP1
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

mRubyFP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

mStrawberryFP
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

tdRFP611
type: Class
subclass of: Red fluorescent proteins from Anthozoa
has OBO namespace: image

PhiYFP
type: Class
subclass of: Yellow fluorescent proteins from Anthozoa
has OBO namespace: image

TagYFP
type: Class
subclass of: Yellow fluorescent proteins from Anthozoa
has OBO namespace: image

TurboYFP
type: Class
subclass of: Yellow fluorescent proteins from Anthozoa
has OBO namespace: image

ZsYellowFP
type: Class
subclass of: Yellow fluorescent proteins from Anthozoa
has OBO namespace: image

fluorescent protein timer
type: Class
subclass of: optical highlighter
has OBO namespace: image

photoactivatable fluorescent protein
type: Class
subclass of: optical highlighter
has OBO namespace: image

photoconvertible fluorescent protein
type: Class
subclass of: optical highlighter
has OBO namespace: image

photoconvertible/photoswitchable fluorescent protein
type: Class
subclass of: optical highlighter
has OBO namespace: image

photoswitchable fluorescent protein
type: Class
subclass of: optical highlighter
has OBO namespace: image

DsRed-ES FP
type: Class
subclass of: fluorescent protein timer
has OBO namespace: image

Fast-FT
type: Class
subclass of: fluorescent protein timer
has OBO namespace: image

Medium-FT
type: Class
subclass of: fluorescent protein timer
has OBO namespace: image

Slow-FT
type: Class
subclass of: fluorescent protein timer
has OBO namespace: image

PA-GFP
type: Class
subclass of: photoactivatable fluorescent protein
has OBO namespace: image

PA-CFP
type: Class
subclass of: photoactivatable fluorescent protein
has OBO namespace: image

PA-mCherry1FP
type: Class
subclass of: photoactivatable fluorescent protein
has OBO namespace: image

PA-mRFP
type: Class
subclass of: photoactivatable fluorescent protein
has OBO namespace: image

Phamret
type: Class
subclass of: photoactivatable fluorescent protein
has OBO namespace: image

Dendra2FP
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

dEOSFP
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

KaedeFP
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

mEOS2FP
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

mKikGR
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

wtEOSFP
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

wtKIikGR
type: Class
subclass of: photoconvertible fluorescent protein
has OBO namespace: image

IrisFP
type: Class
subclass of: photoconvertible/photoswitchable fluorescent protein
has OBO namespace: image

bsDronpa
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

Dronpa
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

Dronpa-3
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

E2GFP
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

KFP1
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

mTFP0.7
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

PadronFP
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

rsCherryFP
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

rsCherryRevFP
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

rsFastLimeFP
type: Class
subclass of: photoswitchable fluorescent protein
has OBO namespace: image

optically-dense stain
type: Class
subclass of: stain with broad specificity
has OBO namespace: image

electron-dense stain
type: Class
subclass of: stain with broad specificity
has OBO namespace: image

uranyl salt
type: Class
subclass of: electron-dense stain
has OBO namespace: image

lead salt
type: Class
subclass of: electron-dense stain
has OBO namespace: image

osmium tetroxide
type: Class
subclass of: electron-dense stain
has OBO namespace: image

potassium permanganate
type: Class
subclass of: electron-dense stain
has OBO namespace: image

uranium compound
type: Class
subclass of: negative staining
has OBO namespace: image

molybdenum compound
type: Class
subclass of: negative staining
has OBO namespace: image

tungsten compound
type: Class
subclass of: negative staining
has OBO namespace: image

aurothioglucose
type: Class
subclass of: negative staining
has OBO namespace: image

other negative stain
type: Class
subclass of: negative staining
has OBO namespace: image

other radioisotope
type: Class
subclass of: metabolically incorporated radioisotope
has OBO namespace: image

isolated subcellular component
type: Class
subclass of: sample preparation method
has OBO namespace: image
has definition: A fragment of a cell, subcellular organelle, or macromolecular complex.
  type: Definition
  has db xref: XX:newdbxref
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_newdbxref

secondary electron generation
type: Class
subclass of: imaged parameter
has OBO namespace: image
has definition: electrons generated by inelastic scattering of other radiation, the primary radiation.
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

critical_point dried specimen
type: Class
subclass of: cryofixed tissue
has OBO namespace: image
has definition: specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions.
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

unprocessed raw data
type: Class
subclass of: computational method
has OBO namespace: image
has definition: data as acquired, with no alterations that change the pixel values.
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

lyophilized specimen
type: Class
subclass of: cryofixed tissue
has OBO namespace: image
has definition: specimen from which water has been removed by evaporation under vacuum at a temperature below freezing.
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

X-Rhodamine
type: Class
subclass of: fluorescent label
has OBO namespace: image
has definition: fluorophore derivative of rhodamine with ex/em 580/605
  type: Definition
  has db xref: XX:NULL
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_NULL

serial block face SEM (SBFSEM)
type: Class
subclass of: back-scattered_electron imaging
has OBO namespace: image
has definition: A method of reconstructing 3D structure by combining serial sectioning with scanning EM.
  type: Definition
  has db xref: PMID_:15514700
    type: DbXref
    has URI: http://purl.org/obo/owl/PMID_#PMID__15514700

elastic scattering of electrons
type: Class
subclass of: elastic scattering
has OBO namespace: image

elastic scattering of photons
type: Class
subclass of: elastic scattering
has OBO namespace: image

inelastic scattering of electrons
type: Class
subclass of: inelastic scattering
has OBO namespace: image

inelastic scattering of photons (Raman scattering)
type: Class
subclass of: inelastic scattering
has OBO namespace: image

source of contrast
type: Class
subclass of: microscopy with lenses
has OBO namespace: image
has definition: The source of the variation in the imaged parameter across the field of view.
  type: Definition
  has db xref: XX:<new_dbxref>
    type: DbXref
    has URI: http://purl.org/obo/owl/XX#XX_<new_dbxref>

differences in chemical composition
type: Class
subclass of: source of contrast
has OBO namespace: image

distribution of epitope
type: Class
subclass of: source of contrast
has OBO namespace: image

form birefringence
type: Class
subclass of: source of contrast
has OBO namespace: image

intrinsic birefringence
type: Class
subclass of: source of contrast
has OBO namespace: image

stress-induced birefringence
type: Class
subclass of: source of contrast
has OBO namespace: image

distribution of a specific nucleic acid sequence
type: Class
subclass of: source of contrast
has OBO namespace: image

distribution of a specific protein
type: Class
subclass of: source of contrast
has OBO namespace: image

differences in adsorption or binding of stain
type: Class
subclass of: source of contrast
has OBO namespace: image

boundaries between regions with different refractive index
type: Class
subclass of: source of contrast
has OBO namespace: image

differences in amount of elastic light scattering
type: Class
subclass of: source of contrast
has OBO namespace: image

differences in deposition of metal shadow
type: Class
subclass of: source of contrast
has OBO namespace: image

differences in intrinsic optical density
type: Class
subclass of: source of contrast
has OBO namespace: image

fluorescence polarization microscopy
type: Class
subclass of: fluorescence microscopy
has OBO namespace: image

compartmentalization of stain or label
type: Class
subclass of: source of contrast
has OBO namespace: image

differences in fluorescence lifetime
type: Class
subclass of: source of contrast
has OBO namespace: image

differences in orientation of fluorophore
type: Class
subclass of: source of contrast
has OBO namespace: image

intrinsic mass distribution
type: Class
subclass of: source of contrast
has OBO namespace: image

X-ray fluorescence
type: Class
subclass of: fluorescence emission
has OBO namespace: image

heat fixed tissue
type: Class
subclass of: sample preparation method
has OBO namespace: image

copper salt
type: Class
subclass of: electron-dense stain
has OBO namespace: image

dispersed cells in vitro
type: Class
subclass of: sample preparation method
has OBO namespace: image

fluorescent protein derived from Arabidopsis
type: Class
subclass of: fluorescent protein tag
has OBO namespace: image

vidicon tube camera
type: Class
subclass of: area detector
has OBO namespace: image

phototropin 2
type: Class
subclass of: fluorescent protein derived from Arabidopsis
has OBO namespace: image

miniSOG
type: Class
subclass of: phototropin 2
has OBO namespace: image

evanescent wave scattering
type: Class
subclass of: dark-field microscopy
has OBO namespace: image
has related synonym: FTIR frustrated total internal reflection

evanescent wave microscopy
type: Class
subclass of: fluorescence microscopy
has OBO namespace: image
has related synonym: total internal reflection fluorescence TIRF

ground state depletion scanning (GSD)
type: Class
subclass of: resolution-enhancing method
has OBO namespace: image

nearfield illumination
type: Class
subclass of: Restriction
  on property: makes_use_of
  some values from: evanescent wave microscopy
has OBO namespace: image

nearfield illumination
type: Class
subclass of: Restriction
  on property: makes_use_of
  some values from: evanescent wave scattering
has OBO namespace: image

freeze-substituted tissue
type: Class
subclass of: cryofixed tissue
has OBO namespace: image

high-voltage electron microscopy (HVEM)
type: Class
subclass of: transmission electron microscopy (TEM)
has OBO namespace: image

intermediate voltage electron microacopy (IVEM)
type: Class
subclass of: transmission electron microscopy (TEM)
has OBO namespace: image

Biological Imaging Method
type: Class
has OBO namespace: image